This family, of recent taxonomic reformulation, includes the genera Agave, Nolina, Yucca and Dasylirion, and is well represented in the collection (about 90 species).

The agave genus, which includes the plants known as magueyes, have popular commercial uses for their juices (aguamiel, pulque and distillates), and their fibers (ixtle, mecates). A large part of this collection is exhibited in the Agave Garden, next to the Conservatory.

The genders Nolina, Yucca Y dasylirion they have several mature specimens in the Garden, mostly from rescues. A kind of the latter Dasylirion acrotriche, It has great ritual relevance among the indigenous communities of the region, and is known as cucharilla or chimal.